FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11567088
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121050.069.64954120
GAGCACA177550.045.515133120
AGAGCAC240650.034.29749118-119
AAGAGCA376350.022.876154120
GAAGAGC406750.020.603594118-119
GGAAGAG510750.016.722153120
TCGGAAG582800.014.762023118-119
CGGAAGA603250.014.305878120
TAGCCCT37600.014.0992832
GCCCTTA42700.013.2162814
GATCGGA697800.012.422871116-117
ATCGGAA705250.012.126549114-115
GTAGCCC43850.011.7071961
TATAGTG43650.011.6299715120
AGATCGG758650.011.245669112-113
TAGATCG147300.010.758221120
AACACTA37450.09.8934667
ACACTAT40350.09.7443238
CACTATA40250.09.4853869
AAGATCG397300.09.309205114-115
Produced by FastQC (version 0.11.5)