FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198022
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC110000.062.379265120
GAGCACA163800.040.991222120
AGAGCAC219400.032.73501120
AAGAGCA350450.021.37135120
GAAGAGC403100.019.152159120
GGAAGAG469600.016.249178118-119
TCGGAAG562700.014.710556120
CGGAAGA570450.013.544819120
GATCGGA658300.012.52559120
ATCGGAA658050.012.316274118-119
AGATCGG736650.011.280342120
TAGATCG190600.010.521295118-119
CTAGATC49100.09.329799118-119
GAGATCG286600.08.986557120
TTAGATC75050.08.964487114-115
AAGATCG260000.08.575301120
CACACGT37750.08.316155120
CAGATCG252750.08.162218120
GTAGATC62800.07.9575334120
CCTAGAT19350.06.953153120
Produced by FastQC (version 0.11.5)