FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16131263
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC203300.098.26224120
GAGCACA338050.058.69332120
AGAGCAC472550.042.449432120
AAGAGCA712100.028.264624118-119
GAAGAGC801850.025.063467118-119
GGAAGAG976750.020.513767116-117
CGGAAGA1130600.017.8687116-117
TCGGAAG1175650.017.039661114-115
ATCGGAA1352750.014.8867235114-115
GATCGGA1395800.014.371495112-113
AGATCGG1524350.013.203957112-113
TAGATCG365250.012.000215110-111
CTAGATC86850.011.089793110-111
GAGATCG493450.010.788181112-113
CACGTCT49850.010.716493120
CACACGT45250.010.641941120
CAGATCG532600.010.490075110-111
AAGATCG498900.010.459629110-111
TTAGATC151450.010.258885114-115
ACACGTC52950.010.231188120
Produced by FastQC (version 0.11.5)