FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15062636
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132700.064.82297120
GAGCACA207250.042.915085120
AGAGCAC280950.032.188892120
AAGAGCA446350.021.031813120
GAAGAGC526750.018.277657120
GGAAGAG615050.016.265827120
TCGGAAG696650.014.467799118-119
CGGAAGA717450.014.242854120
ATCGGAA829150.012.582494120
GATCGGA838450.012.342272120
AGATCGG934100.011.252198120
TAGATCG235950.09.836305114-115
CTAGATC59700.09.406483116-117
TTAGATC91900.08.4416685114-115
AAGATCG383600.08.038942120
GAGATCG402550.07.4992366120
CAGATCG373750.06.843851116-117
TAAGATC143350.06.793248120
CCTAGAT23750.06.6971188116-117
CACACGT38300.06.2717233120
Produced by FastQC (version 0.11.5)