FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13815543
Sequences flagged as poor quality0
Sequence length51-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA233850.08.4410305120
AAAAAAA634050.08.270368120
TGGTCGC366300.07.714733120
CGGTGGT481050.07.5956335120
GGTCGCC375550.07.492763120
GTGGTCG402250.07.4279714120
GTATCAT246850.07.413164120
AGATCTC620400.07.1951203120
CTCGGTG544150.07.1228013120
TCTCGGT560800.07.082505120
TCGGTGG503850.07.037576120
TAGATCT637950.06.987778120
GGTGGTC429900.06.894399120
GATCTCG630950.06.7990465120
ATCTCGG620150.06.733632120
CGCCGTA349100.06.702729120
TGTAGAT799650.06.6176767120
GAGTGTA850150.06.59156120
GTGTAGA795400.06.51726120
TCATTAA198500.06.4985256120
Produced by FastQC (version 0.11.5)