FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16126488
Sequences flagged as poor quality0
Sequence length53-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA20150.011.6120411
GATCCAT49850.010.8316951
GATCCTG71750.09.8668631
GATCCTC52400.09.8465971
TAGATCT336750.09.780981120
GATCCCT20000.09.5992881
GTGGTCG191550.09.333655120
AGATCTC324500.09.133347120
GAGTGTA516100.09.055684120
GATCCTT50500.09.0290331
GATCCGT41250.08.8720681
TGGTCGC173200.08.867675120
GATCTAC32600.08.83371
AGTGTAG477150.08.763862120
AAGAGTG626100.08.662483120
GATCATA49700.08.4500771
TCTCGGT290950.08.433787120
GATCTCG333850.08.428284120
TCGGTGG250200.08.344679120
GATCTTA38150.08.3348991
Produced by FastQC (version 0.11.5)