FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13432598
Sequences flagged as poor quality0
Sequence length65-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG85250.010.218623120
GGTGGTC420300.08.705158120
GTGGTCG384200.08.459772120
TGGTCGC351950.08.432663120
AAAAAAA669100.08.161907120
TAGATCT659550.07.70622120
GGTCGCC351400.07.5910172120
CTCGGTG525650.07.5205226120
TCGGTGG492050.07.4846244120
GATCTCG632700.07.416038120
CGGTGGT475050.07.375538118-119
AGATCTC643450.07.3201504120
ATCTCGG584200.07.1678677120
CGCCGTA315750.07.135209120
TCTCGGT545500.07.0389104118-119
CGTATCA248000.07.0156274118-119
TCGCCGT371750.06.8199353120
GTAGATC809950.06.7499714120
GTGTAGA821400.06.6924505120
GCCGTAT308900.06.690511120
Produced by FastQC (version 0.11.5)