FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13853416
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121600.056.13366120
GAGCACA186950.037.12644120
AGAGCAC230850.029.59807118-119
GAAGAGC384000.018.823242120
AAGAGCA393750.018.308493120
GGAAGAG501300.014.476297118-119
TCGGAAG555150.013.38819120
CGGAAGA578450.013.091763120
GATCGGA642900.011.749531120
ATCGGAA683550.011.120584118-119
AGATCGG691100.011.087141120
TAGATCG159950.010.410224118-119
AAGATCG355550.09.37452120
CTAGATC45100.08.636395120
TTAGATC81600.08.248315114-115
GAGATCG225450.08.19223118-119
CAGATCG206650.06.9522653120
TAAGATC169100.06.5702953120
CAAGATC219100.06.23125118-119
GCTAGAT24150.05.8118396108-109
Produced by FastQC (version 0.11.5)