FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15146711
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC103700.041.369225120
GAGCACA148200.032.242443120
AGAGCAC180900.024.996109118-119
AAGAGCA319700.015.874394120
GAAGAGC336800.014.407685120
TCGGAAG435000.012.547814120
GGAAGAG422500.011.953415118-119
GATCGGA501000.011.857205120
CGGAAGA498200.011.439942120
AGATCGG555350.010.808094120
ATCGGAA534700.010.797751120
TAGATCG137450.010.613629120
CTAGATC33200.08.37855116-117
TTAGATC64050.07.431359120
GAGATCG261800.07.0835056120
AAGATCG258450.06.76872120
CAGATCG256250.06.296124118-119
TGAGATC117700.05.251945120
ATAGATC103100.05.126301118-119
CCTAGAT17551.0919393E-85.1072025114-115
Produced by FastQC (version 0.11.5)