FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12102294
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC91700.051.971508120
GAGCACA135250.035.707954120
AGAGCAC176950.029.597448120
GAAGAGC292050.017.903572118-119
AAGAGCA306700.017.159288120
GGAAGAG361300.014.198982116-117
TCGGAAG390600.013.147277120
CGGAAGA420850.012.575734116-117
GATCGGA442500.011.419819112-113
ATCGGAA481200.010.9782915114-115
AGATCGG496100.010.474817112-113
TAGATCG115350.010.103994116-117
CACGTCT33600.09.101961120
CACACGT24700.08.512361120
CTAGATC32650.08.484966114-115
GAGATCG219050.07.766065120
AAGATCG199100.07.7442102120
TTAGATC62300.07.3633842120
ACACGTC31500.06.9752913118-119
CAGATCG189500.06.721192112-113
Produced by FastQC (version 0.11.5)