FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12937849
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC201050.0139.0509120
GAGCACA347900.079.99321120
AGAGCAC464200.059.87578118-119
AAGAGCA691550.038.948486118-119
GAAGAGC767100.036.2614116-117
GGAAGAG939650.028.721575116-117
CCGATCT86000.025.7938694
CGGAAGA1122950.024.822025114-115
TCCGATC91350.024.4451163
TCGGAAG1151950.023.368595114-115
CACGTCT56550.022.191288120
ACACGTC57050.022.181644120
ATCGGAA1335950.020.966927112-113
CGATCTA25250.020.3036615
CACACGT49550.020.11321118-119
GATCGGA1351850.019.975294112-113
AGATCGG1441900.019.429705110-111
GCACACG63000.018.580208120
CTTCCGA123650.018.5544991
ACGTCTG76150.017.725897120
Produced by FastQC (version 0.11.5)