FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18243666
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC223100.081.85318120
GAGCACA369100.049.08511120
AGAGCAC490900.037.252098118-119
AAGAGCA761100.023.899311118-119
GAAGAGC821450.022.112778116-117
GGAAGAG996750.019.123526120
TCGGAAG1160150.016.23753120
CGGAAGA1132700.016.138298114-115
ATCGGAA1386800.014.035869120
GATCGGA1408100.013.6268118-119
AGATCGG1558700.012.030468118-119
TAGATCG374050.011.242139116-117
CACGTCT61900.011.060162120
CTAGATC89350.010.56587120
CACACGT54650.010.417547120
GAGATCG662550.09.588296116-117
TTAGATC111700.09.290572112-113
CAGATCG572400.09.190962116-117
ACACGTC62950.09.10142118-119
AAGATCG455750.08.459713120
Produced by FastQC (version 0.11.5)