FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14125002
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC202400.093.523186120
GAGCACA326400.054.725292120
AGAGCAC443000.042.11414120
AAGAGCA673200.028.88147120
GAAGAGC762400.025.032558118-119
GGAAGAG909450.021.117723118-119
CGGAAGA1055500.018.211424116-117
TCGGAAG1099850.017.423973116-117
ATCGGAA1286600.015.038711114-115
GATCGGA1318700.014.660826114-115
AGATCGG1448000.013.3818112-113
CACACGT57750.012.539372120
TAGATCG351850.012.094882116-117
CTAGATC83100.011.759464118-119
ACACGTC65700.011.6146345120
CACGTCT63100.011.352809120
GAGATCG517450.010.836696112-113
TTAGATC141900.010.674727110-111
CAGATCG503850.010.487726112-113
GCACACG79700.010.160612120
Produced by FastQC (version 0.11.5)