FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12361675
Sequences flagged as poor quality0
Sequence length61-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG17450.018.413078120
TCTAGGT18350.017.86733120
CTAGGTG14150.017.527685114-115
CTAGGGA11650.015.0235195108-109
AAAAAAA1178450.014.617552120
TCTAGGG15650.013.526667116-117
GTCTAGG15650.013.440467114-115
CGTCTAG21100.013.052467120
ATCTAGG17250.012.921805112-113
GTCTAGA22350.011.799284114-115
GATCTAG20550.011.305084112-113
CTCAGTG30750.010.022556120
CTAGGGC7451.8189894E-129.902225116-117
GAGTCTA25800.09.613051112-113
CGTATCA516550.09.21616120
TAGCTCT41750.09.109557120
GTATCAT507500.08.876596120
ATCATTA459900.08.854294120
CCGTATC592650.08.807255120
GTAGTGT37250.08.765183114-115
Produced by FastQC (version 0.11.5)