FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16076533
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169700.074.4311120
GAGCACA258050.049.868454120
AGAGCAC344950.037.99435120
AAGAGCA539300.024.422749118-119
GAAGAGC581050.022.384314118-119
GGAAGAG738700.018.416594120
CGGAAGA878100.015.222219118-119
TCGGAAG883900.015.200372118-119
GATCGGA1061500.013.603166120
ATCGGAA1056500.013.333835120
AGATCGG1190250.011.951302118-119
CTAGATC67250.011.225854118-119
TAGATCG261050.011.009982120
AAGATCG539300.09.450758118-119
GAGATCG411700.09.419952120
CACACGT49000.09.384999120
TTAGATC115200.09.314317118-119
TAAGATC224750.08.25235116-117
CAGATCG341900.08.227017118-119
GCTAGAT30800.07.4653397120
Produced by FastQC (version 0.11.5)