FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12903586
Sequences flagged as poor quality0
Sequence length57-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT144250.010.730537120
AGTGTAG209100.09.439727120
GAGTGTA227450.09.126574120
AGATCTC150100.09.033277120
AAGAGTG294250.09.032435120
GTAGGGA448900.08.84761120
GTGTAGA216050.08.775066120
TGTAGGG481900.08.727276120
AGAGTGT252500.08.506271120
TAGGGAA431000.08.254581120
GTGTAGG533150.08.169676120
CGTCGTG709800.08.021356120
TCGTGTA601750.07.9063253120
GTCGTGT633850.07.903466120
CGTGTAG562200.07.8329043120
AGCGTCG794450.07.7934675120
CTCGGTG127050.07.744382120
TGGTCGC90900.07.6561728120
GTAGATC229900.07.620114118-119
GATCTCG159100.07.5041327120
Produced by FastQC (version 0.11.5)