FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742691
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208450.073.4628120
GAGCACA327800.046.238323120
AGAGCAC446800.035.780006120
AAGAGCA674950.023.388268118-119
GAAGAGC750650.020.85301118-119
GGAAGAG897500.017.406525116-117
CGGAAGA1062950.015.244465120
TCGGAAG1070600.014.763974118-119
ATCGGAA1274800.012.785772120
GATCGGA1271900.012.707999120
CACGTCT62500.011.750184120
AGATCGG1400850.011.569764118-119
TAGATCG281400.010.777001114-115
CACACGT59950.010.662024120
AAGATCG595050.010.20467120
CTAGATC73050.09.86672114-115
ACACGTC67500.09.670934118-119
GAGATCG457250.09.145604116-117
GTCTAGG5050.0063989859.1070322
TTAGATC118250.08.97066120
Produced by FastQC (version 0.11.5)