FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16457273
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT68900.016.4569264
TCCGATC78100.015.5944813
GCGTCGG59550.014.708711120
AAAAAAA1877150.013.720357120
CGATCTA23500.010.9775835
CGCCGTA1050750.09.295195120
CTTCCGA153600.09.2184371
GGTCGCC1297750.09.21802120
CGTATCA876200.09.19552120
GTCGCCG1219850.09.024708120
GCCGTAT1015350.09.01068120
TCATTAA677250.08.929252120
ATCATTA769350.08.897443120
TCGCCGT1138800.08.755624120
GTATCAT849400.08.744035120
TGGTCGC1391400.08.735564120
GTGGTCG1502450.08.597012120
CATTAAA662050.08.400255120
CCGTATC975550.08.338994120
GGTGGTC1614200.08.150511120
Produced by FastQC (version 0.11.5)