FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12316774
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC119250.061.30436120
GAGCACA180000.041.970387120
AGAGCAC236350.034.504272120
AAGAGCA375950.021.345266118-119
GAAGAGC426050.018.773306118-119
GGAAGAG522150.015.444366116-117
TCGGAAG617900.013.47782114-115
CGGAAGA647550.013.044361116-117
ATCGGAA728100.011.58287114-115
GATCGGA723800.011.578345112-113
AGATCGG790500.010.655638112-113
ACACGTC61300.010.332864120
TAGATCG190600.010.208416110-111
CTAGATC42750.09.180944114-115
CACGTCT56900.09.158711118-119
CACACGT61450.09.126558120
TTAGATC67200.08.149272120
GAGATCG364500.08.116248112-113
ACGTCTG75200.07.984239120
CAGATCG332350.07.7110744118-119
Produced by FastQC (version 0.11.5)