FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083164
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC104350.054.978077120
GAGCACA152450.036.182796120
AGAGCAC202000.029.963007120
AAGAGCA329450.018.869694120
GAAGAGC380550.016.178228118-119
GGAAGAG472100.013.889919120
TCGGAAG516150.013.169155120
GATCGGA592500.012.558667120
CGGAAGA564250.011.5432005120
ATCGGAA620250.011.528725120
AGATCGG655250.011.230774120
TAGATCG153650.010.659094118-119
CTAGATC39500.010.305966116-117
TTAGATC66950.09.096874118-119
AAGATCG316650.08.769833120
GAGATCG247450.08.518775120
GCTAGAT18150.07.302319120
TAAGATC125350.07.3006544120
CAGATCG230600.07.2527833120
TCTAGAT22700.06.95076120
Produced by FastQC (version 0.11.5)