FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12876823
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC174250.098.35877120
GAGCACA284650.058.287785120
AGAGCAC388900.043.502876118-119
AAGAGCA595300.029.554676120
GAAGAGC684450.026.200792120
GGAAGAG835000.021.355488118-119
CCGATCT68250.020.0639424
TCCGATC69700.019.4107843
CGGAAGA1000900.018.564053120
CGATCTA17800.018.1556133
TCGGAAG1023600.017.435766116-117
ATCGGAA1180350.015.126837118-119
GATCGGA1206450.014.768563114-115
AGATCGG1319400.013.5005116-117
CTTCCGA108250.012.9151491
TAGATCG333750.012.440816112-113
CTAGATC81450.012.069962110-111
GAGATCG444150.011.718136112-113
GATCTAG7051.3256795E-811.6541626
AAGATCG393650.011.045597118-119
Produced by FastQC (version 0.11.5)