FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13815674
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178350.078.40083120
GAGCACA289950.048.414227120
AGAGCAC389400.036.128147118-119
AAGAGCA592250.023.863909118-119
GAAGAGC657600.022.014942120
GGAAGAG818550.018.273209120
CGGAAGA949800.015.596317120
TCGGAAG971350.015.476442120
ATCGGAA1146650.013.144552118-119
GATCGGA1164200.012.931687118-119
ACACGTC51100.011.8212805120
CACACGT45300.011.819501120
AGATCGG1276400.011.816396116-117
TAGATCG276900.011.161082118-119
CTAGATC71850.010.395669118-119
AAGATCG598850.010.029811120
CCTAGAT23300.010.016726120
TTAGATC114850.09.86179120
CACGTCT49450.09.7170515120
GAGATCG390850.09.466326120
Produced by FastQC (version 0.11.5)