FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14709401
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC172950.071.90188120
GAGCACA272250.047.896015120
AGAGCAC365550.036.0659120
AAGAGCA570200.024.771334120
GAAGAGC651300.021.866034120
GGAAGAG795850.017.933413118-119
TCGGAAG1056350.015.028731120
CGGAAGA1041500.014.4320755120
CACGTCT99550.013.174508120
ATCGGAA1232600.012.7015120
CACACGT107800.012.548442120
GATCGGA1262650.012.508032118-119
ACGTCTG113850.012.122917118-119
CGTCTGA124850.011.604761120
ACACGTC110800.011.495804116-117
AGATCGG1400200.011.217992118-119
TGAACTC99150.010.526724120
TAGATCG316050.010.493864118-119
CTAGATC74100.010.146806114-115
GAACTCC107500.09.900692120
Produced by FastQC (version 0.11.5)