FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13407365
Sequences flagged as poor quality0
Sequence length60-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG165000.09.456557120
TAGGGAA327750.08.752435120
TGTAGGG386150.08.470015120
AAGAGTG226700.08.418201120
GAGTGTA178100.08.289244120
CGTGTAG456100.08.184158120
TCGTGTA480400.08.144947120
GTAGGGA345700.08.089659118-119
AGAGTGT197450.07.993598120
GTGTAGG425850.07.863597120
GTCGTGT517500.07.723383120
TAGATCT123400.07.61102118-119
GTGTAGA176150.07.563347120
GTAGATC193900.07.45904120
AAAGAGT271000.07.329973120
GAGCGTC749800.07.2434726120
AGATCTC145350.07.2254806120
TTAGATC78350.07.200001120
CGTCGTG618050.07.020344120
AGCGTCG699050.06.970942120
Produced by FastQC (version 0.11.5)