FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682822
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185700.065.52435120
GAGCACA283800.044.72187120
AGAGCAC383600.034.077446120
AAGAGCA604350.022.139608120
GAAGAGC678350.019.65143118-119
GGAAGAG840150.016.206583120
CGGAAGA981100.014.365802120
TCGGAAG977950.014.170867120
GATCGGA1156700.012.485146120
ATCGGAA1147700.012.446031120
AGATCGG1288950.011.539646120
TAGATCG343450.010.616757118-119
CTAGATC87200.09.692779116-117
GAGATCG483650.09.591904120
TTAGATC137350.09.326279118-119
AAGATCG460200.08.585409118-119
CAGATCG437550.08.086653120
CCTAGAT30450.07.5310435112-113
ATAGATC162050.07.48041120
CACGTCT72500.07.230273120
Produced by FastQC (version 0.11.5)