FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12756137
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA149000.010.830996120
GTAGGGA308450.010.016704120
AGAGTGT175400.09.919066120
TGTAGGG334450.09.847898120
TCGTGTA444550.09.59514120
CGTGTAG405650.09.55396120
GTGTAGG377650.09.499791120
AGTGTAG135450.09.434154120
GTCGTGT471600.09.413698120
TAGGGAA295650.09.212561120
GTGTAGA149400.08.79419120
AGCGTCG610950.08.788601120
TAGATCT108000.08.77679120
GAGCGTC665350.08.674157120
AAGAGTG216000.08.582368120
GCGTCGT571750.08.394332120
CGTCGTG534200.08.333019120
AGAGCGT726750.07.9165497120
AAGAGCG773050.07.527773120
GAAGAGC876900.07.5188255120
Produced by FastQC (version 0.11.5)