FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13056190
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG78800.013.098128120
AAAAAAA736400.09.91708120
GGTGGTC546100.08.944565120
GGTCGCC453300.08.909152120
TGGTCGC465700.08.852329120
GTGGTCG503850.08.539352120
GTCGCCG443200.08.35396120
CGCCGTA377900.08.33661120
CTCGGTG682200.08.32121120
AGATCTC877100.08.312549120
ATCTCGG788250.08.290313120
CGGTGGT622900.08.246363120
TAGATCT928650.08.135434120
TCTCGGT728750.08.127288120
GATCTCG833650.08.011584120
GCCGTAT381450.07.944394120
TCGCCGT422500.07.9394813120
TCGGTGG651400.07.8302965120
CGTATCA327200.07.684361120
AGTGTAG1233600.07.4328613120
Produced by FastQC (version 0.11.5)