FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12268067
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG70900.010.832068120
AAAAAAA796250.010.232882120
GTGGTCG463400.08.480723120
TGGTCGC425150.08.425191120
CGTATCA295700.08.197429120
CGCCGTA349200.08.023988120
GGTGGTC500700.08.016709120
CCCCCCG7000.0073387097.71422152
ATCTCGG698800.07.684538120
CTCGGTG617150.07.6706805120
GGTCGCC411000.07.66417120
GATCTCG737050.07.6357822120
TCGCCGT404850.07.558291120
AGATCTC774950.07.4404182120
GCCGTAT348800.07.3795257120
TCGGTGG585450.07.3788776120
GTCGCCG409950.07.3764477120
TAGATCT801850.07.2955675120
CATTAAA261050.07.2629156120
CGGTGGT558750.07.1677256118-119