FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12268067
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136400.081.11891120
GAGCACA233450.046.093525120
AGAGCAC306450.035.735348118-119
AAGAGCA491450.023.048613120
GAAGAGC552800.020.303137120
GGAAGAG659050.017.512302120
CGGAAGA743750.015.85248120
TCGGAAG752800.015.464377118-119
ATCGGAA884600.013.453379120
GATCGGA898300.013.27128120
AGATCGG987700.012.069687118-119
TAGATCG250600.011.113968120
CTAGATC70950.010.422611120
AAGATCG312200.09.875135118-119
GAGATCG353800.09.680066118-119
TTAGATC92500.09.554825118-119
CCTAGAT24550.09.289827120
CAGATCG327700.09.260508116-117
CCCCCCC6203.750706E-49.2132551
TGAGATC165900.08.7898445120
Produced by FastQC (version 0.11.5)