FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13001399
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC144300.060.51972120
GAGCACA212250.042.59775120
AGAGCAC284700.032.56416120
AAGAGCA453850.021.482836120
GAAGAGC500700.018.974752120
GGAAGAG617450.015.631503118-119
CGGAAGA730900.013.941151120
TCGGAAG701450.013.839108118-119
ATCGGAA860250.012.355932120
AGATCGG947250.011.789084120
GATCGGA864050.011.709292120
TAGATCG208500.010.179645116-117
CTAGATC56150.09.7566595116-117
CACACGT44400.09.753178120
TTAGATC80950.09.361706118-119
CACGTCT45400.09.104789120
AAGATCG375450.08.598014120
ACACGTC48850.08.461769120
GAGATCG363350.08.378726120
CAGATCG369350.07.9762373118-119
Produced by FastQC (version 0.11.5)