FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852282
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136150.070.37639120
GAGCACA212750.043.431347120
AGAGCAC285350.033.719814120
AAGAGCA443400.021.987505120
GAAGAGC510950.019.513407120
GGAAGAG596300.017.664297120
CGGAAGA694450.015.196661120
TCGGAAG706850.014.943725118-119
ATCGGAA829650.013.075566120
GATCGGA854500.012.435681116-117
AGATCGG947350.011.34452118-119
TAGATCG237300.011.012234120
CTAGATC58050.09.926698120
CACACGT42300.09.187476120
TTAGATC96700.08.5572195118-119
AAGATCG343050.08.535571120
GAGATCG391100.08.283251118-119
AGAGATC128900.07.641402120
TAAGATC123950.07.6222143120
CAGATCG334000.07.512473114-115
Produced by FastQC (version 0.11.5)