FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19214370
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC200550.060.968815120
GAGCACA291100.041.11714120
AGAGCAC391450.031.318327120
AAGAGCA621150.021.346987120
GAAGAGC681100.018.79328118-119
GGAAGAG843950.015.98669120
TCGGAAG965900.013.875423118-119
CGGAAGA1009850.013.270915120
ATCGGAA1165300.011.993388120
GATCGGA1159700.011.990101120
AGATCGG1277850.010.984576118-119
TAGATCG258950.010.109997118-119
CTAGATC73250.09.777697120
AAGATCG611200.08.571968118-119
ACACGTC76000.08.150399120
CACACGT61650.08.148316118-119
TTAGATC114050.08.131555118-119
GAGATCG435550.07.844291118-119
CACGTCT67150.07.30279120
CAGATCG447400.07.2398396120
Produced by FastQC (version 0.11.5)