FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15837571
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG386550.012.0279045120
TAGGGAA254300.011.889897120
TCGTGTA424600.011.599976120
GTAGGGA271100.011.595667120
GTGTAGG354550.011.506026120
TGTAGGG312500.011.21132120
GTCGTGT460950.011.192795120
GAGTGTA112650.010.8640785120
AGAGTGT133250.010.535195120
AGTGTAG102900.010.435941120
AGCGTCG630300.010.165246120
CGTCGTG545450.09.975766120
GCGTCGT587300.09.581572120
GAGCGTC706850.09.02194120
AAGAGCG836950.08.916919120
AAGAGTG171100.08.800709120
AGAGCGT773850.08.744727120
GAAGAGC945500.08.26755120
TCGGAAG1106500.07.937497120
GTGTAGA127100.07.9297166120
Produced by FastQC (version 0.11.5)