FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15346646
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC227400.0101.17897120
GAGCACA370700.061.31751120
AGAGCAC503050.045.46062118-119
AAGAGCA763500.030.445265120
GAAGAGC851550.026.874968116-117
GGAAGAG1037100.022.015507118-119
CGGAAGA1226250.018.719494114-115
TCGGAAG1259550.018.062704116-117
ATCGGAA1473750.015.71412112-113
GATCGGA1521850.015.094184114-115
AGATCGG1638700.014.121915110-111
CACGTCT58950.013.855113120
CACACGT56400.013.757463120
CTAGATC119900.012.88283108-109
TAGATCG378900.012.865279112-113
CAGATCG721000.011.672999110-111
ACACGTC64500.011.523266120
TTAGATC135050.011.19675112-113
GAGATCG524150.010.9797325112-113
AAGATCG435150.010.002993110-111
Produced by FastQC (version 0.11.5)