FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14733309
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC229100.073.236015120
GAGCACA349750.048.960323120
AGAGCAC465650.036.426327118-119
AAGAGCA681500.025.348537120
GAAGAGC746350.022.461279120
GGAAGAG913450.019.140366120
ACACGTC56650.016.771872120
CGGAAGA1045150.016.570076120
CACACGT58500.016.364521120
TCGGAAG1065200.016.177094118-119
CACGTCT59250.015.306987120
ATCGGAA1295750.013.64315120
GATCGGA1339350.013.207086116-117
AGATCGG1478700.011.923532116-117
ACGTCTG80650.011.245369120
TAGATCG273750.010.833048116-117
CTAGATC67200.010.550489108-109
CGTCTGA93750.09.674016120
GCACACG94600.09.625137118-119
GAGATCG570150.09.279098120
Produced by FastQC (version 0.11.5)