FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12115802
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC143450.068.604996120
GAGCACA226650.044.98319120
AGAGCAC296400.033.689713120
AAGAGCA457600.023.111277120
GAAGAGC517500.021.221695120
GGAAGAG632700.017.176605118-119
CGGAAGA745850.014.803559120
TCGGAAG760950.014.58735120
ATCGGAA902400.013.092763120
GATCGGA921400.013.064718120
CACACGT42000.012.020347120
AGATCGG1037050.011.592119118-119
TAGATCG220000.011.146296118-119
CTAGATC58400.010.441231118-119
ACACGTC49050.010.0253105120
CACGTCT46550.09.859478120
TTAGATC82900.09.807148120
AAGATCG411250.09.055615120
GAGATCG395400.08.597926118-119
CAGATCG364900.08.498903120
Produced by FastQC (version 0.11.5)