FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11863009
Sequences flagged as poor quality0
Sequence length57-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC258500.09.306235120
AGTGTAG340650.08.946342120
TAGATCT254800.08.852758118-119
TGTAGAT362700.08.650562120
GTGTAGA337550.08.299826120
AGAGTGT404550.08.274699120
TCTCGGT219900.08.184388120
GAGTGTA371400.08.1733265120
AAGAGTG446450.07.7668552120
GATCTCG283750.07.759261120
GTAGATC348250.07.648602120
GTAGGGA671100.07.5894556120
ATCTCGG245700.07.5203104120
TCGGTGG200600.07.44661120
TGGTCGC144050.07.41305120
GTGTAGG776300.07.356946120
TGTAGGG716650.07.3163505120
TAGGGAA640750.07.302914120
CGGTGGT212650.07.222121120
CTCGGTG227300.07.2053227120
Produced by FastQC (version 0.11.5)