FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11863009
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC114950.061.578120
GAGCACA183800.041.50593120
AGAGCAC240950.031.900352120
AAGAGCA378950.021.718933120
GAAGAGC425150.019.449112120
GGAAGAG504400.016.437878118-119
TCGGAAG594950.014.011372118-119
CGGAAGA616300.013.790668120
ATCGGAA715650.012.546899120
GATCGGA726450.012.545376120
AGATCGG827500.011.523852120
TAGATCG157750.011.116309120
CCTAGAT14350.09.811834120
CTAGATC39000.09.353963116-117
GAGATCG382750.08.402417118-119
CAGATCG354550.08.104911120
TTAGATC59600.07.9463716118-119
CACACGT34700.07.838678118-119
AAGATCG280000.07.679991120
ACACGTC47650.07.118563120
Produced by FastQC (version 0.11.5)