FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11808430
Sequences flagged as poor quality0
Sequence length64-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG92200.010.169705120
GGTCGCC382450.010.1210575120
GTGGTCG450400.09.5149355120
TGGTCGC399500.09.373158120
CTCGGTG605800.09.356149120
GATCTCG770150.09.3496685120
TAGATCT844600.09.25139120
TCTCGGT640800.09.126515120
ATCTCGG705250.08.906107120
AAAAAAA614550.08.841498120
CGTATCA240950.08.7807255120
GGTGGTC485050.08.773269120
CGCCGTA296500.08.757371120
TCGGTGG581650.08.690583120
CGGTGGT537900.08.559372120
AGATCTC800100.08.533923120
GTAGATC1046400.08.357568120
GCCGTAT290950.08.284012120
GTCGCCG401300.08.252721120
GTGTAGA1089650.08.252001120
Produced by FastQC (version 0.11.5)