FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13249673
Sequences flagged as poor quality0
Sequence length54-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13950.016.91243120
AGTCTAG13350.011.290789120
TCTAGGG10700.09.280326106-107
TCTAGGT12908.074494E-99.144511120
CGTAGTG45100.09.009337120
AGATCTC295400.08.275153120
TAGATCT297450.08.240154120
GAGTGTA429300.08.060291120
GTGGTCG189850.07.905013120
AGTGTAG396400.07.803439120
GTAGATC389200.07.6783824120
CTCGGTG269400.07.638529120
GATCTCG291700.07.616246120
ATCTCGG290550.07.308056120
AGAGTGT473000.07.287898120
GTGTAGA401200.07.122021120
GATCTAG11056.566552E-107.116547118-119
GGTGGTC212250.07.039873120
TCTCGGT280400.06.988289120
TGGTCGC176500.06.9430017118-119
Produced by FastQC (version 0.11.5)