FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056276
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC117950.073.262146120
GAGCACA189250.044.47007120
AGAGCAC257950.036.222927120
AAGAGCA389250.023.15316120
GAAGAGC447600.020.801096120
GGAAGAG528150.017.421621118-119
TCGGAAG630700.015.161545120
CGGAAGA629150.014.761861116-117
ATCGGAA737500.013.154642120
GATCGGA754850.012.992749120
AGATCGG847350.011.801206120
TAGATCG213950.011.088447118-119
CTAGATC54900.010.622209114-115
TTAGATC84200.09.719794116-117
AAGATCG321550.08.635108116-117
GAGATCG336550.08.575088118-119
CAGATCG283850.07.84423118-119
CCTAGAT22500.07.510306118-119
GCCTAGA8659.416035E-76.8948746118-119
CACGTCT32000.06.8338265120
Produced by FastQC (version 0.11.5)