FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17068783
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167850.061.04738120
GAGCACA245350.040.13753120
AGAGCAC326100.032.27099120
AAGAGCA538050.020.69516120
GAAGAGC578900.019.379345120
GGAAGAG715200.016.316072120
TCGGAAG829450.014.8989725120
CGGAAGA845450.014.327718120
GATCGGA1023050.012.803033120
ATCGGAA1004350.012.2083120
AGATCGG1122900.011.324161118-119
TAGATCG248150.010.846542118-119
CTAGATC59100.09.619387118-119
TTAGATC107250.08.821965120
AAGATCG509000.08.4241295118-119
GAGATCG438650.07.732818120
CAGATCG431700.07.24602120
CCTAGAT24350.06.463376116-117
GCTAGAT32150.06.2062035120
TAAGATC192750.06.010678120
Produced by FastQC (version 0.11.5)