FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16548289
Sequences flagged as poor quality0
Sequence length55-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG101300.09.830734120
AAAAAAA1185800.09.116553120
TGGTCGC621950.08.35314120
CGTATCA407650.08.182286120
CGCCGTA518550.08.144593120
GTGGTCG666000.08.079892120
GTCGCCG614850.08.039803120
GGTGGTC717650.07.9414215120
GGTCGCC592650.07.9259405120
GTAGGGG28300.07.8433685120
TCGGTGG833800.07.8352585120
GCCGTAT493550.07.8156877120
GATCTCG1097350.07.741149120
ATCTCGG1013500.07.7364044120
CTCGGTG884550.07.6570044120
GTATCAT440650.07.5422916120
AGATCTC1138150.07.511086120
TCTCGGT943250.07.479419120
CCGTATC468500.07.4780884120
TCGCCGT583250.07.415968120