FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11482565
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130900.062.437145120
GAGCACA198250.044.872986120
AGAGCAC262250.032.43324118-119
AAGAGCA403600.021.730198118-119
GAAGAGC457650.020.962076120
GGAAGAG568700.016.516907120
TCGGAAG674100.015.083607120
CGGAAGA677000.014.113087120
GATCGGA803950.012.751768120
ATCGGAA792900.012.644691118-119
AGATCGG910100.011.888686120
CACACGT42750.010.872899120
CACGTCT45700.010.736095120
TAGATCG205000.010.549847118-119
CTAGATC53300.09.871555118-119
TTAGATC79000.09.724534120
GAGATCG347750.09.356477120
AAGATCG343400.09.305803120
CAGATCG307050.08.683402120
ACACGTC50900.08.117291120
Produced by FastQC (version 0.11.5)