FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14046656
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC128350.060.851517120
GAGCACA196700.039.771343120
AGAGCAC249900.033.2914120
AAGAGCA410950.021.034563120
GAAGAGC464550.018.922735120
GGAAGAG563850.015.513047118-119
TCGGAAG666650.013.443969120
CGGAAGA672800.013.425149120
GATCGGA763550.013.271753120
ATCGGAA776500.012.222468120
AGATCGG838850.011.6684065118-119
TAGATCG216100.010.882907118-119
CTAGATC50350.010.08698112-113
GAGATCG340400.09.3872175120
TTAGATC88400.09.083601116-117
AAGATCG264100.08.990071120
CAGATCG306400.08.55916120
TCTAGAT31250.07.9439645120
GCCTAGA8400.00260138937.577805120
AGAGATC116250.07.398883116-117
Produced by FastQC (version 0.11.5)