FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14326497
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC199850.0100.404755120
GAGCACA330200.058.47573120
AGAGCAC443100.044.464897118-119
AAGAGCA663300.029.455158118-119
GAAGAGC754150.026.300762116-117
GGAAGAG932450.021.094893116-117
CGGAAGA1074550.018.578321114-115
TCGGAAG1107200.017.765474114-115
ATCGGAA1288550.015.548322112-113
GATCGGA1323900.014.88423112-113
AGATCGG1429350.013.971542110-111
TAGATCG394600.012.822793110-111
CTAGATC85850.012.265208110-111
GAGATCG574100.011.309209120
CAGATCG523150.010.732086110-111
CACACGT47900.010.426556120
TTAGATC120150.010.3694935112-113
CCTAGAT29700.09.742939110-111
ATAGATC197200.09.538028112-113
CACGTCT50650.09.443071118-119
Produced by FastQC (version 0.11.5)