FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293331
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC177400.0115.94293120
GAGCACA293050.069.439606120
AGAGCAC399300.051.237534118-119
AAGAGCA598550.033.874104118-119
GAAGAGC684850.030.008104116-117
GGAAGAG830750.024.421875116-117
CGGAAGA977050.021.109919114-115
TCGGAAG1005850.020.153072114-115
ATCGGAA1156900.017.859802112-113
GATCGGA1186800.017.05951112-113
AGATCGG1276500.016.06924110-111
TAGATCG312350.014.687018110-111
CTAGATC81150.013.649364108-109
GAGATCG468200.013.10782110-111
CAGATCG445200.012.605112110-111
AAGATCG326500.012.428262110-111
TTAGATC109550.012.185011112-113
TGAGATC200850.011.923285108-109
CCTAGAT19550.010.97311108-109
ATAGATC129750.010.930789110-111
Produced by FastQC (version 0.11.5)