FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696801
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152650.071.101875120
GAGCACA236400.046.58433120
AGAGCAC321600.035.00437120
AAGAGCA484350.023.270012118-119
GAAGAGC560600.020.505991120
GGAAGAG675400.016.741531118-119
CGGAAGA795350.014.778104120
TCGGAAG804150.014.599924120
ATCGGAA941900.012.816047120
GATCGGA950050.012.504296118-119
AGATCGG1061400.011.659946120
TAGATCG252800.011.099996120
CTAGATC57150.011.001234120
CACGTCT44900.09.433388120
AAGATCG374750.09.271536120
GAGATCG432150.09.18863120
TTAGATC94850.08.5474825114-115
CAGATCG350650.08.248004118-119
TAAGATC134050.07.8994055118-119
TGAGATC157400.07.82077118-119
Produced by FastQC (version 0.11.5)