FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16284778
Sequences flagged as poor quality0
Sequence length54-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2864450.014.49643120
TCTCGGG40000.013.499447120
GCGTCGG101800.013.4965120
CTAGAGG7802.154049E-610.0187551
CGTATCA1235900.09.374161120
TCATTAA966300.09.0899725120
GTATCAT1181200.08.965092120
ATCATTA1060250.08.952239120
TATCATT1199550.08.917979120
CATTAAA935100.08.905635120
CGCCGTA1577250.08.669166120
CCGTATC1381050.08.5409775120
GCCGTAT1475000.08.2532215120
TGGTCGC2014000.07.9271827120
GTCGCCG1835550.07.834933120
GGTCGCC1904700.07.774134120
TCGCCGT1733650.07.734809120
GGTGGTC2302700.07.6680756120
GTGGTCG2155200.07.4245286120
AAAGAGG196100.07.2205095120
Produced by FastQC (version 0.11.5)