FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14391011
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160050.069.244354120
GAGCACA252250.044.251854120
AGAGCAC335400.034.11576120
AAGAGCA513600.022.265892118-119
GAAGAGC580750.020.597906120
GGAAGAG717550.016.485193120
TCGGAAG822600.014.631097120
CGGAAGA838500.014.294047118-119
GATCGGA973450.012.617098120
ATCGGAA961400.012.5293865116-117
AGATCGG1077350.011.2642145120
TAGATCG291450.010.655398118-119
TTAGATC109050.010.022266120
CTAGATC68700.09.6036005116-117
GAGATCG405600.08.954603118-119
CCTAGAT23950.08.904146120
AAGATCG359050.08.834864120
CAGATCG350300.08.209114116-117
ATAGATC145150.07.3919096120
GCTAGAT26400.07.320651116-117
Produced by FastQC (version 0.11.5)